Reduced Volume Formol Titration for Fermentable Nitrogen


The full equation for calculating assimilable nitrogen is:

mg N/L = (mL of NaOH) x (0.05meq OH-/mL) x (1mmol N/meq OH-) x (25mL/10mL- dilution factor) x 1000/10 (to convert to liters) x 14 mg/mmol N

If a different concentration of base is used, the equation requires an additional term: (0.05/N of NaOH used). The dilution factor in the equation (25 mL/10 mL) is changed if one uses a sample volume other than 10 mL and dilution to 25 mL.

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